This book constitutes the refereed proceedings of the 12th Enterprise Engineering Working Conference on Advances in Enterprise Engineering XVI, EEWC 2022, held in Leusden, The Netherlands, November 2–3, 2022. EEWC aims at addressing the challenges that modern and complex enterprises are facing in a rapidly changing world. The participants of the working conference share a belief that dealing with these challenges requires rigorous and scientific solutions, focusing on the design and engineering of enterprises. The goal of EEWC is to stimulate interaction between the different stakeholders, scientists as well as practitioners, interested in making Enterprise Engineering a reality. The 4 full papers and 2 short papers included in this book were carefully reviewed and selected from 13 submissions. In addition, there are 2 invited papers from keynote presentations. They were organized in topical sections as follows: Invited Papers from Keynote Presentations and Presented Papers.