This book discusses various biofabrication approaches, processes, and new technologies developed over the years. Technologies covered by the volume include 3D (bio)printing, non-printing manufacturing, hybrid biofabrication, innovations in biomaterials for biofabrication, physicochemical, rheological and mechanical properties of bioinks, translation of physical cues into cellular responses, smart material systems, PRP/PL-based personalized materials, biofabrication strategies for neovascularization, biofabrication with polymers, ceramics and composites, pre-clinical applications for skin, tendons, trachea, bone, skeletal muscle, blood vessels, ocular tissues, 3D-engineered platforms for disease modeling, deep learning, artificial intelligence in biofabrication, and more. It is clear that these approaches will ultimately lead to the development of functional products that may contain living (cells or tissues) and/or non-living (supporting proteins, biomaterials) components. Leading scientists provide insightful perspectives on approaches to this inherently interdisciplinary field, shedding light on future developments and potential new applications. This is an ideal resource for biomedical and bioengineering researchers working at different stages of development of regenerative medicine tools, including tissue engineering, biomaterials, stem cells, cell therapy and cell banking; scientific administrators and graduate students in biotechnology, cell and molecular biology, materials science and engineering and chemistry fields.