This book offers a thorough overview of the rapidly expanding field of biophotonic manipulation, delving into topics such as the fundamentals of optical forces, technologies of optical manipulation, and their applications in the biomedical field. The recent recognition of Arthur Ashkin with the Nobel Prize for his groundbreaking work on optical tweezers has sparked a renewed interest and importance in the realm of optical manipulation. In response to this, the authors present a timely and comprehensive book that focuses on the basics and uses of various optical manipulation technologies, catering to a readership with a strong interest in this advancing field. This book not only enhances readers’ current knowledge base but also serves as a valuable resource for researchers, scientists, and enthusiasts looking to gain a deeper understanding of the transformative power of optical manipulation.