This book introduces the reader in the fast-developing, fascinating field of Electrochemiluminescence (ECL) imaging and ECL microscopy (ECLM) with its outstanding ability to visualize micro-objects, such as bio-conjugated magnetic beads, cellular membranes, subcellular components, tissues, polypeptide interactions and nucleic acid structures, with unprecedented sensitivity and spatial and temporal resolution. The reader is provided with the most recent fundamental and instrumental aspects of ECLM, including the strategies for signal enhancement and approaches to super-resolution and mechanistic investigations aimed at highlighting hidden ECL which may eventually facilitate the enhancement of its bioanalytical performance. By including contributions from the principal authors worldwide in the field of ECLM and ECL imaging, this book offers a breadth of coverage and depth of treatment to this new exciting field. It provides a comprehensive view of ECLM as a reference text for advanced researchers. At the same time, the fundamentals of ECL are also described so as to make it fully accessible to beginners and students. In doing this, the authors put their best efforts to describe the processes in the most straightforward way while never neglecting the rigor that distinguish the physical-chemistry of the electrogenerated chemiluminescence phenomena. The final result is a multidisciplinary resource that encompasses electrochemistry, material chemistry, physics, chemistry and biology that sets the reference, from fundamentals to application, for a wide range of scientist in an effort to foster this fascinating research into new and creative pathways.