Genetic Library Construction and Sc - E-book

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Genetic Library Construction And Screening - ENG

Advanced Techniques And Applications

R. Curtis Bird; Bruce F. Smith

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Sinopsis

ISBN:	9783642564086
Formato:	Page Fidelity
Idioma:	Inglés
Editorial:	Springer Nature
Tema:	Ciencia
Subtema:	Ciencias de la Vida biología molecular
Año de publicación:	2012-12-06

Designed as an introductory text the authors cover all core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies. The handbook contains detailed step-by-step protocols and extensive hands-on advice.

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